Journal: Molecular Medicine Reports

Article Title: Effects of combined treatment with PD-L1 Ig and CD40L mAb on immune tolerance in the CBA/J × DBA/2 mouse model

doi: 10.3892/mmr.2020.10977

Figure Lengend Snippet: Expansion of the CD4 + CD25 + T-cell population is driven by combined treatment with PD-L1 Ig and anti-CD40L mAbs in the CBA/J × DBA/2 model. (A) Number of CD4 + CD25 + T cells in splenocytes was determined by flow cytometry. (B) Gating strategy used for flow cytometry, using the FSC/SSC method. R1 represents the T lymphocyte population. (C) Percentage of CD4 + CD25 + T cells. ***P<0.001 vs. the normal group (CBA/J × BALB/c). ### P<0.001 vs. the spontaneous abortion group (CBA/J × DBA/2); &&& P<0.001 vs. PD-L1 Ig + CD40L mAb group. PD-L1, programmed death-ligand 1; Ig, immunoglobulin; CD40L, CD40 ligand; mAb, monoclonal antibody; FSC, forward scatter; SSC, side scatter; PE, phycoerythrin.

Article Snippet: The splenic cells were labeled with both anti-mouse CD25 FITC (cat. no. BG-07312-50-100; BioGems) and anti-mouse CD4 PE (cat. no. 85-12-0041-81; eBioscience; Thermo Fisher Scientific, Inc.) in the dark at 4°C for 30 min.

Techniques: Flow Cytometry

Journal: PLoS ONE

Article Title: Ustekinumab Improves Psoriasis without Altering T Cell Cytokine Production, Differentiation, and T Cell Receptor Repertoire Diversity

doi: 10.1371/journal.pone.0051819

Figure Lengend Snippet: The percentage of nTreg (FoxP3 + CD127 low CD25 high CD4 + T cells/CD4 + T cells) was similar among the seven volunteers. Flow cytometry data from four patients and three healthy controls are shown.

Article Snippet: For identification of nTreg (FoxP3 + CD127 low CD25 high CD4 + T cells), magnetically collected CD4 + T cells during the third blood sampling were directly stained with monoclonal antibodies to CD127-FITC and CD25-PE in cell surface staining buffer containing 0.1M PBS and 2% FCS (Biowest, Nuaillé, France).

Techniques: Flow Cytometry